Purification and properties of D-glycerate 3-kinase from Escherichia coli.

نویسندگان

  • C C Doughty
  • J A Hayashi
  • H L Guenther
چکیده

The adaptive D-glycerate 3-kinase from glycerate-adapted Escherichia coli has been purified 4200-fold in 37% yield and has been crystallized. The procedure, starting with the crude, cell-free extract, consisted of treatment with protamine sulfate, ammonium sulfate, passage through a diethylaminoethyl Sephadex column, and precipitation with ethanol. The reaction product, 3-phosphoglycerate, has been identified by paper chromatography and specific enzymatic assay. Some characteristics of the enzyme which show clearly its differences from other glycerate kinases are described.

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عنوان ژورنال:
  • The Journal of biological chemistry

دوره 241 3  شماره 

صفحات  -

تاریخ انتشار 1966